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. 2019 Mar 22;26(12):2594–2606. doi: 10.1038/s41418-019-0322-9

Fig. 2.

Fig. 2

MEST is required for transformation and migration of triple-negative breast cancer cells. a Western blot analysis of MEST expression in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. β-actin was used as a loading control. b Formation of spheroid colonies of 4T1 control-shRNA or MEST-shRNA cells. Spheroid colonies in suspension were then photographed at ×200 magnification. c The soft agar colony-forming assay of the 4T1 control-shRNA or MEST-shRNA cells (n = 3). P < 0.0001, t-test. d The migration assay of in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells (n = 3). P < 0.0001, t-test. e Wound healing assay of Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. Wound closures were photographed at 0 and 24 h after wounding (magnification: ×200)