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. 2019 Mar 22;26(12):2594–2606. doi: 10.1038/s41418-019-0322-9

Fig. 3.

Fig. 3

High MEST expression induces EMT program. a Western blot analysis of the expression of E-cadherin, N-cadherin, fibronectin, and vimentin proteins in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. β-actin was used as a loading control. b Western blot (left) and RT-PCR (Right) analysis of the expression of CAR, caludin3, occludin, E-cadherin, α-catenin, N-cadherin, fibronectin, and β-catenin proteins in MCF10A-control or MCF10A-MEST cells. GAPDH and β-actin were used as a loading control. c Immunofluorescence images of E-cadherin and N-cadherin in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. The red signal represents staining of the corresponding protein, while the blue signal represents DAPI staining. d Immunofluorescence images of V5, E-cadherin, β-catenin, fibronectin, α-catenin, and N-cadherin in MCF10A-control or MCF10A-MEST cells. The red signal represents the staining of the corresponding protein, while the blue signal represents DAPI staining