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. 2019 Mar 22;26(12):2594–2606. doi: 10.1038/s41418-019-0322-9

Fig. 5.

Fig. 5

MEST led to Twist-1 upregulation through activation of STAT3. a Western blot analysis of the expression of phospho-JAK2, JAK2, phospho-STAT3, STAT3, and Twist-1 proteins in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. β-actin was used as a loading control. b Western blot analysis of the expression of phospho-JAK2, JAK2, phospho-STAT3, STAT3, and Twist-1 proteins in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells treated with or without 20 ng/ml IL-6. β-actin was used as a loading control. c Western blot analysis of the expression of phospho-STAT3 and STAT3 proteins in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells after nuclear fractionation. Lamin B1 was used as a nuclear loading control. d Immunofluorescence images of phospho-STAT3 and STAT3 in Hs578T, SUM159PT, and 4T1 control-shRNA or MEST-shRNA cells. The green signal represents the staining of the corresponding protein, while the blue signal represents DAPI staining