Fig. 6.

MEST induces STAT3 activation through induction of JAK2–STAT3 complex formation. a Identification of the complex formation of endogenous MEST/STAT3 in Hs578T, SUM159PT and 4T1 cells. b Western blot analysis of the whole-cell lysates and immunoprecipitates derived from 293T cells transfected with the V5-MEST and Flag-STAT3 constructs, as indicated. c  Identification of the STAT3 region that interacted with MEST. Western blot analysis of whole-cell lysates and immunoprecipitates derived from 293T cells transfected with V5-MEST, Flag-STAT3 wild, and the STAT3 deletion construct (STAT3 mt) as indicated. d Western blot analysis of the whole-cell lysates and immunoprecipitates derived from 293T cells transfected with the V5-MEST, V5-MESTΔC, and Flag-STAT3 constructs, as indicated. e Western blot analysis of the expression of phospho-STAT3 and STAT3 proteins in Hs578T, SUM159PT, and 4T1 MEST-shRNA cells after transient transfection of MEST or MESTΔC constructs. β-actin was used as a loading control. f Western blot analysis of whole-cell lysates and immunoprecipitates derived from 293T cells after transfection of JAK2, STAT3, or MEST constructs. β-actin was used as a loading control. g Identification of the complex formation of endogenous JAK2/STAT3 in Hs578T and SUM159PT control-shRNA or MEST-shRNA cells. h Western blot analysis of whole-cell lysates and immunoprecipitates derived from 293T cells after transfection of JAK2, STAT3, MEST, or MESTΔC constructs. β-actin was used as a loading control