Fig. 3.

ZIKV induces programmed cell death of hESC-derived NPCs. a Annexin V-FITC/PI staining followed by flow cytometry analyses of cell apoptosis in mock and ZIKV groups (MOI=0.006 for 6 days). Q1, dead cells and cell debris. Q2, late apoptotic cells. Q3, early apoptotic cells. Q4, living cells. b Confocal images stained with ZIKV envelope proteins in green and cleaved Caspase 3 in red of mock or ZIKV-infected FD, FV and H&S organoids at 3 dpi. Scale bar, 100 μm. c Percentage of cleaved Caspase 3 positive cells in mock and ZIKV-infected groups. Data are presented as mean ± SEM. n = 3. Unpaired two-tailed Student’s t-test. **p<0.01 compared to mock controls