Fig. 4.

ZIKV infection induces antiviral immune response through activation of ISGs. a Principal component analysis of global RNA sequencing from mock (round) and MOI = 0.5 ZIKV-infected (triangle) FD, FV, H&S organoids at 3 dpi or 6 dpi. b Upregulated genes in ZIKV-infected organoids at 6 dpi compared to mock groups. 28 genes were significantly upregulated in all three ZIKV-treated regional organoids. c List of Gene Ontology (GO) classification of the common 28 upregulated genes and the corresponding number. d Heatmaps of 28 upregulated genes in ZIKV-infected FD, FV and H&S organoids. The RPKM values were log2-transformed and row-scaled. e qRT-PCR analysis of mRNA expression of 8 ISGs of MOI = 0.5 ZIKV infected vs mock infected FD, FV and H&S organoids at 3 dpi. Data are presented as mean ± SEM. n = 3. Unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01 compared to mock controls