Table 1.
Overview of our TCRαβ-Jurkat mining and functional validation. Clone and donor identifiers, pMHC targets, pMHC dextramer panning TCRβ sequence frequencies, cellular activation TCRβ frequencies, and monoclonal validation data for TCRαβ clones identified with our technology. “ND” stands for not determined. “+” indicates a binder, “-“ indicates a non-binder.
| TCRD Read Frequency (%) | TCRD Read Frequency (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Clone ID | Donor | Target | Starting Library | Post-Panning | Dextramer Enrichment | Input | Activated Cells | Activation Enrichment | Monoclonal Binding | Monoclonal EC50 (nM) |
| NLV.1 | CSS-930 | pp65(NLV) | 0.000035% | 47.61% | 1,359,118 | 33.47% | 35.08% | 1.048 | Not expressed | ND |
| NLV.2 | CSS-930 | pp65(NLV) | 0.090000% | 46.54% | 517 | 28.89% | 23.89% | 0.827 | + | No activation |
| NLV.3 | CSS-930 | pp65(NLV) | 0.000035% | 2.64% | 75,364 | 16.70% | 0.04% | 0.002 | − | ND |
| NLV.4 | CSS-930 | pp65(NLV) | 0.000035% | 0.54% | 15,415 | 0.95% | 0.003% | 0.003 | − | ND |
| NLV.5 | CSS-938 | pp65(NLV) | 0.090000% | 72.86% | 810 | ND | 1 ND I | ND | − | ND |
| NLV.6 | CSS-938 | pp65(NLV) | 0.000557% | 6.45% | 11,577 | ND | ND | ND | − | ND |
| NLV.7 | CSS-938 | pp65(NLV) | 0.010000% | 3.98% | 398 | ND | 1 ND 1 | ND | − | ND |
| NLV.8 | CSS-944 | pp65(NLV) | 0.000043% | 96.76% | 2,254,385 | 6.59% | 79.74% | 12.100 | + | 6.616 |
| NLV.9 | CSS-944 | pp65(NLV) | 0.000172% | 1.53% | 8,912 | 21.36% | 1.65% | 0.077 | + | No activation |
| NLV.10 | CSS-944 | pp65(NLV) | 0.000300% | 0.97% | 3,229 | 0.12% | 0.75% | 6.250 | + | 0.267 |
| VLE.1 | CSS-930 | IE-1 (VLE) | 0.030000% | 44.54% | 1,485 | 36.51% | 63.22% | 1.732 | + | 4.650 |
| VLE.2 | CSS-930 | IE-1 (VLE) | 0.000035% | 15.93% | 454,752 | 9.16% | 4.35% | 0.475 | − | ND |
| VLE.3 | CSS-930 | IE-1 (VLE) | 0.000841% | 14.92% | 17,747 | 6.98% | 3.94% | 0.564 | Not expressed | ND |
| VLE.4 | CSS-930 | IE-1 (VLE) | 0.160000% | 5.49% | 34 | 3.45% | 2.92% | 0.846 | − | ND |
| GLC.1 | CSS-930 | BMLF1 (GLC) | 0.160000% | 85.49% | 534 | 78.56% | 88.64% | 1.128 | + | 38.895 |
| GLC.2 | CSS-930 | BMLF1 (GLC) | 0.000035% | 3.61% | 103,054 | 3.25% | 2.43% | 0.748 | − | ND |
| GLC.3 | CSS-930 | BMLF1 (GLC) | 0.002697% | 0.85% | 315 | 2.95% | 0.27% | 0.092 | − | ND |
| GLC.4 | CSS-930 | BMLF1 (GLC) | 0.000490% | 0.51% | 1,040 | 0.84% | 0.06% | 0.071 | − | ND |
| GLC.5 | CSS-930 | BMLF1 (GLC) | 0.030000% | 0.38% | 13 | 0.50% | 0.02% | 0.040 | Not expressed | ND |
| GLC.6 | CSS-938 | BMLF1 (GLC) | 0.002340% | 92.46% | 39,513 | 35.47% | 76.86% | 2.167 | Weak | 591.950 |
| GLC.7 | CSS-938 | BMLF1 (GLC) | 0.000260% | 2.95% | 11,346 | 27.76% | 0.03% | 0.001 | − | ND |
| GLC.8 | CSS-938 | BMLF1 (GLC) | 0.003714% | 1.77% | 477 | 14.30% | 0.005% | 0.000 | − | ND |
| CLG.1 | CSS-930 | LMP2(CLG) | 0.020000% | 64.75% | 3,238 | 46.44% | 83.95% | 1.808 | + | 0.355 |
| CLG.2 | CSS-930 | LMP2(CLG) | 0.001857% | 12.03% | 6,480 | 9.35% | 1.75% | 0.187 | − | ND |
| CLG.3 | CSS-930 | LMP2(CLG) | 0.000035% | 8.14% | 232,372 | 4.73% | 0.82% | 0.173 | − | ND |
| CLG.4 | CSS-930 | LMP2(CLG) | 0.002522% | 3.27% | 1,297 | 1.26% | 6.60% | 5.238 | Weak | 25.595 |
| CLG.5 | CSS-930 | LMP2(CLG) | 0.008477% | 0.73% | 86 | 0.75% | 0.06% | 0.080 | − | ND |
| CLG.6 | CSS-944 | LMP2(CLG) | 0.460000% | 29.03% | 63 | 0.27% | 18.54% | 68.667 | − | No activation |
| CLG.7 | CSS-944 | LMP2(CLG) | 0.003863% | 33.35% | 8,633 | 0.29% | 26.06% | 89.862 | Weak | 0.668 |
| CLG.8 | CSS-944 | LMP2(CLG) | 0.001245% | 25.27% | 20,302 | 0.25% | 15.32% | 61.280 | − | No activation |
| CLG.9 | CSS-944 | LMP2(CLG) | 0.002833% | 11.45% | 4,042 | 0.04% | 5.84% | 146.000 | − | No activation |
| TYG.1 | CSS-948 | LMP2(TYG) | 0.009477% | 97.58% | 10,297 | 8.99% | 92.71% | 10.313 | Weak | 2.516 |
| TYG.2 | CSS-948 | LMP2(TYG) | 0.060000% | 0.20% | 3 | 0.01% | 0.0065% | 0.653 | − | ND |
| TYG.3 | CSS-948 | LMP2(TYG) | 0.060000% | 0.26% | 4 | 3.67% | 0.0065% | 0.00178 | − | ND |
| PMEL.1 | CSS-799 | PMEL(KTW) | 0.120000% | 87.35% | 728 | 4.26% | 64.23% | 15.077 | + | 14.505 |
| PMEL.2 | CSS-799 | PMEL(KTW) | 0.001963% | 1.43% | 728 | 0.44% | 6.23% | 14.159 | + | 13.547 |
| PMEL.3 | CSS-799 | PMEL(KTW) | 0.001963% | 0.03% | 15 | 0.004% | 0.56% | 158.222 | + | 11.967 |
| PMEL.4 | CSS-799 | PMEL(KTW) | 0.300000% | 1.77% | 6 | 0.58% | 5.43% | 9.362 | + | 12.840 |
| PMEL.5 | CSS-799 | PMEL(KTW) | 0.300000% | 4.62% | 15 | 0.46% | 0.94% | 2.043 | + | 5.148 |
ND = Not Determined
− = No Dextramer Binding
+ = Dextramer Binding