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. 2020 May 14;11(5):369. doi: 10.1038/s41419-020-2580-3

Fig. 2. TDP-43 transcriptional activity on CHOP promoter.

Fig. 2

a CHOP-luciferase plasmid was co-transfected with WT or mutant TDP-43 in SH-SY5Y cells and luciferase activity was measured, in a multiplate reader using the Dual-GlowTM Luciferase Assay System (Promega, USA). Firefly luciferase activity was then normalized to the Renilla luciferase activity to control the transfection efficiency. Data were then normalized to luciferase activity in cells transfected with empty vector, which was given a value of 100%. The data were obtained from four independent experiments; **p > 0.01 and ***p > 0.001, analysed by using one-way ANOVA with Bonferroni’s Multiple comparison post-hoc test. b ChIP assay by anti-H3-PS10- AcK14H3 on CHOP promoter upon treatment with sodium arsenite (10 µM) for 1, 3, or 6 h, using EZ-Magna ChIP™ (Millipore), according to the manufacturer’s protocol. c SHSY5Y cells were transduced with TDP-43 and 24 h postinfection cells were fixed, lysed and used for ChIP analysis. d quantification of ChIP experiment by using the Fold Enrichment Method. The data were obtained from four independent experiments; **p > 0.015 for the fold enrichment, analysed by using one-way ANOVA with Bonferroni’s Multiple comparison post-hoc test.