Fig. 9.
Tsc1/Rheb/mTORC1 does not affect mTORC2 signaling activation in kidney fibroblasts. a, b The primary cultured kidney fibroblasts generated from Rhebfl/fl, or Tsc1fl/fl mice were infected with adeno-Cre virus to induce Rheb, or Tsc1 gene deletion. Western blotting assay showing that ablation of Rheb or Tsc1 could not affect the p-Akt (Ser473) abundance. c, d Western blot analyses showing p-Akt (Ser473) abundance in the Sham and IRI kidneys. Numbers indicate each individual animal within each group. e Real-time qRT-PCR analysis showing Raptor mRNA abundance in NRK-49F cells transfected with scramble or Raptor siRNA as indicated. *P < 0.05 versus cells transfected with scramble siRNA, n = 3. n = 3 refers to three independent repeats. f Western blotting analyses showing that knocking down Raptor expression inhibited S6 phosphorylation but not Akt phosphorylation at Ser473. g Western blot analyses showing the abundance of p-S6 and p-Akt (Ser473) in NRK-49F cells after rapamycin treatment