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. 2020 May 14;15(5):e0228510. doi: 10.1371/journal.pone.0228510

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© 2020 Karakaş et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Isolated cells from bone marrow stroma of healthy donors display typical hMSC phenotype and mesodermal differentiation capacity (A, B) After isolation of human bone marrow cells from healthy donors, cells at passage 3 were analyzed by flow cytometry for phenotypic characterization. The cells showed hMSC specific marker expressions with negative expression of CD14, CD31, CD34, and CD45, while they were positive for CD29, CD44, CD73 and CD105. Representative histograms and surface antigen profiles of isolated adherent cells from 5 healthy donors are demonstrated. (C) Adipogenic differentiation was confirmed by Oil Red-O staining of lipid vacuoles (with hematoxylin counterstain); osteogenic differentiation was followed with Toluidine Blue staining of calcium deposits showing mineralization; and Alcian Blue staining of proteoglycans in chondrogenic pellet demonstrated chondrogenic differentiation.