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. 2020 May 14;15(5):e0233169. doi: 10.1371/journal.pone.0233169

Fig 4. Effect of dietary GSE supplementation on in vitro steroidogenesis at basal state (A) and in response to IGF1 and LH alone or in combination in hen primary granulosa cells.

Fig 4

Granulosa cells from preovulatory follicles 1 (F1) from different group of animals (A to D) were seeded for 24 h and after overnight serum starvation, granulosa cells were incubated with IGF1 (10-8M), LH (10-8M) or both for 48 h. The culture medium was then collected. Basal progesterone level (A) and the response to IGF1 or/and LH (B) were assessed. A: animals fed with control diet without GSE supplementation, B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old, and D: supplementation with GSE at 1% of the total diet composition starting at hatch until 40 week-old. Results are presented as lsmeans ± S.E.M. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05. **P < 0.005 and **** P < 0.0001.