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. 2020 May 14;15(5):e0233169. doi: 10.1371/journal.pone.0233169

Fig 5. Level of Reactive Oxygen Species (ROS, H202) in egg yolk of broiler hens fed with different concentrations of GSE dietary supplementation.

Fig 5

ROS level (H202) was assessed from 1 g of each egg yolk using Ros-Glo assay. Ten egg yolks were analysed for each group of animals. A: animals fed with control diet without GSE supplementation, B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old (experiment 1), and D: supplementation at 1% of the total diet composition starting at the hatching until 40 week-old (experiment 2). Results are presented as lsmeans ± S.E.M. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05. **** P < 0.0001.