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. 2020 May 14;15(5):e0233169. doi: 10.1371/journal.pone.0233169

Table 2. Percentage of unfertilised eggs, early (EEM) and late (LEM) embryonic mortality and fertility after artificial insemination in broiler hens fed with GSE supplementation.

Parameters A B C D Diet ABC Diet AD
% Unfertilised 5.89 ± 0.89 a 4.23 ± 0.65 b 4.06 ± 0.75 b 6.28 ± 0.99 0.40 0.98
% EEM 4.62 ± 0.69 4.49 ± 0.55 5.46 ± 0.65 3.89 ± 0.54 0.16 0.73
% LEM 1.29 ± 0.47 1.2 ± 0.28 1.33 ± 0.33 1.12 ± 0.37 0.98 0.81
% Fertility 88.8 ± 1.19 90.53 ± 0.76 89.73 ± 1.11 89.17 ± 1.15 0.93 0.92
% Hatchability of incubated eggs 79.88 ± 1.04 79.55 ± 0.99 79.87 ± 1.15 77.5 ± 1.38 0.99 0.67
% Hatchability of fertile eggs 90.05 ± 0.64 87.93 ± 1.01 89.05 ± 0.83 86.86 ± 0.88 0.94 0.6

Results are presented as lsmeans ± SEM. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05. EEM, early embryo mortality; LEM, late embryo mortality. A: animals fed with control diet without GSE supplementation, B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old and D: supplementation at 1% of the total diet composition starting at hatch until 40 week-old.