Table 3. Progesterone, testosterone, androstenedione and oestradiol concentrations in egg yolk at 33 weeks-old.

Period	Group	Progesterone (ng/g)	Testosterone (ng/g)	Androstenedione (ng/g)	Oestradiol (ng/g)
33 weeks	A	3846 ± 53.47 a	3.3 ± 0.06 a	540.5 ± 5.26 a	4.66 ± 0.07 a
	B	2839 ± 63.82 b	2.21 ± 0.04 b	235.2 ± 4.45 b	4.15 ± 0.13 b
	C	2827 ± 45.1 b	1.69 ± 0.06 c	243.4 ± 6.21 b	4.58 ± 0.06 a
	D	2679 ± 42.47	2.07 ± 0.05	271.2 ± 7.54	4.33 ± 0.06
P	Diet ABC	<0.0001	<0.0001	<0.0001	0.0003
P	Diet AD	<0.0001	<0.0001	<0.0001	0.001

Results are presented as lsmeans ± SEM. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05. Thirty eggs yolk were collected from broiler hens fed with different concentrations of GSE dietary supplementation or with a control diet. A: animals fed with control diet without GSE supplementation, B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old (experiment 1) and D: supplementation at 1% of the total diet composition starting at hatch until 40 week-old (experiment 2).