Table 5. mRNA expression of adipokines (RARRES2, ADIPOQ, NAMPT) and their receptors (CMKLR1, CCRL2, GPR1, ADIPOR1, ADIPOR2) in granulosa and theca cells from preovulatory follicle 1 (F1).
Tissues | Genes | A | B | C | D | Diet ABC | Diet AD |
---|---|---|---|---|---|---|---|
Granulosa cells F1 | RARRES2 | 0.4 ± 0.09 | 0.32 ± 0.03 | 0.33 ± 0.04 | 0.3 ± 0.04 | 0.56 | 0.32 |
CMKLR1 | 0.001 ± 2.10-4a,b | 0.0014 ± 3.10-4a | 5.10−4 ± 5.10-5b | 6.10−4 ± 3.10−4 | 0.04 | 0.31 | |
CCRL2 | 0.003 ± 7.10-4a | 0.003 ± 6.10-4a | 6.10−4 ± 1.10-4b | 0.001 ± 7.10−4 | 0.02 | 0.2 | |
GPR1 | 0.5 ± 0.12 a | 0.3 ± 0.03 a,b | 0.22 ± 0.02 b | 0.19 ± 0.02 | 0.01 | 0.03 | |
ADIPOR1 | 0.002 ± 4.10−4 | 0.002 ± 2.10−4 | 0.003 ± 5.10−4 | 0.001 ± 2.10−4 | 0.6 | 0.01 | |
ADIPOR2 | 0.008 ± 7.10−4 | 0.008 ± 6.10−4 | 0.01 ± 0.001 | 0.007 ± 0.001 | 0.05 | 0.66 | |
NAMPT | 0.02 ± 0.002 a | 0.01 ± 0.001 b | 0.01 ± 0.001 b | 0.009 ± 0.001 | 0.0003 | 0.0002 | |
Theca cells F1 | RARRES2 | 0.41 ± 0.04 | 0.4 ± 0.04 | 0.49 ± 0.03 | 0.36 ± 0.03 | 0.19 | 0.33 |
CMKLR1 | 0.11 ± 0.03 | 0.12 ± 0.02 | 0.16 ± 0.04 | 0.08 ± 0.02 | 0.51 | 0.38 | |
CCRL2 | 0.45 ± 0.1 | 0.49 ± 0.06 | 0.77 ± 0.11 | 0.45 ± 0.03 | 0.06 | 0.98 | |
GPR1 | 0.1 ± 0.02 | 0.14 ± 0.02 | 0.08 ± 0.01 | 0.05 ± 0.006 | 0.23 | 0.05 | |
ADIPOQ | 0.18 ± 0.05 | 0.21 ± 0.04 | 0.33 ± 0.07 | 0.19 ± 0.05 | 0.14 | 0.88 | |
ADIPOR1 | 5.10−4 ± 1.10−4 | 5.10−4 ± 3.10−4 | 1.10−4 ± 4.10−5 | 4.10−4 ± 2.10−4 | 0.16 | 0.85 | |
ADIPOR2 | 0.002 ± 4.10−4 | 0.001 ± 3.10−4 | 0.001 ± 3.10−4 | 0.001 ± 4.10−4 | 0.45 | 0.48 | |
NAMPT | 0.13 ± 0.03 | 0.16 ± 0.02 | 0.12 ± 0.02 | 0.09 ± 0.01 | 0.47 | 0.14 |
Results are presented as lsmeans ± SEM. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05.