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. 2020 May 14;15(5):e0233169. doi: 10.1371/journal.pone.0233169

Table 5. mRNA expression of adipokines (RARRES2, ADIPOQ, NAMPT) and their receptors (CMKLR1, CCRL2, GPR1, ADIPOR1, ADIPOR2) in granulosa and theca cells from preovulatory follicle 1 (F1).

Tissues Genes A B C D Diet ABC Diet AD
Granulosa cells F1 RARRES2 0.4 ± 0.09 0.32 ± 0.03 0.33 ± 0.04 0.3 ± 0.04 0.56 0.32
CMKLR1 0.001 ± 2.10-4a,b 0.0014 ± 3.10-4a 5.10−4 ± 5.10-5b 6.10−4 ± 3.10−4 0.04 0.31
CCRL2 0.003 ± 7.10-4a 0.003 ± 6.10-4a 6.10−4 ± 1.10-4b 0.001 ± 7.10−4 0.02 0.2
GPR1 0.5 ± 0.12 a 0.3 ± 0.03 a,b 0.22 ± 0.02 b 0.19 ± 0.02 0.01 0.03
ADIPOR1 0.002 ± 4.10−4 0.002 ± 2.10−4 0.003 ± 5.10−4 0.001 ± 2.10−4 0.6 0.01
ADIPOR2 0.008 ± 7.10−4 0.008 ± 6.10−4 0.01 ± 0.001 0.007 ± 0.001 0.05 0.66
NAMPT 0.02 ± 0.002 a 0.01 ± 0.001 b 0.01 ± 0.001 b 0.009 ± 0.001 0.0003 0.0002
Theca cells F1 RARRES2 0.41 ± 0.04 0.4 ± 0.04 0.49 ± 0.03 0.36 ± 0.03 0.19 0.33
CMKLR1 0.11 ± 0.03 0.12 ± 0.02 0.16 ± 0.04 0.08 ± 0.02 0.51 0.38
CCRL2 0.45 ± 0.1 0.49 ± 0.06 0.77 ± 0.11 0.45 ± 0.03 0.06 0.98
GPR1 0.1 ± 0.02 0.14 ± 0.02 0.08 ± 0.01 0.05 ± 0.006 0.23 0.05
ADIPOQ 0.18 ± 0.05 0.21 ± 0.04 0.33 ± 0.07 0.19 ± 0.05 0.14 0.88
ADIPOR1 5.10−4 ± 1.10−4 5.10−4 ± 3.10−4 1.10−4 ± 4.10−5 4.10−4 ± 2.10−4 0.16 0.85
ADIPOR2 0.002 ± 4.10−4 0.001 ± 3.10−4 0.001 ± 3.10−4 0.001 ± 4.10−4 0.45 0.48
NAMPT 0.13 ± 0.03 0.16 ± 0.02 0.12 ± 0.02 0.09 ± 0.01 0.47 0.14

Results are presented as lsmeans ± SEM. P values and different individual letters indicate a significant effect of the diet between A, B and C groups (experiment 1) or between A and D groups (experiment 2). P value was considered as significant if P < 0.05.