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. 2020 May 14;15(5):e0232825. doi: 10.1371/journal.pone.0232825

Table 1. Disinfection methods and protocols used in this study.

Compound Active substance Application method Concentration Application protocol
Formaldehyd Biozid 20% (Jäklechemie, Nürnberg, Germany) Formaldehyde Fumigation 44 ml/m3 15 min exposure time, 10 min neutralization time with ammonia, 300 min ventilation
Wessoclean® K50 Goldline (Bio-Clean B.V., Arnhem, Netherlands) Hydrogen peroxide/acid mix Aerosol (particle size 5–10 μm) Hydrogen peroxide: 0.5 ml/m3, ethanol: 500 ml/m3, propan-2-ol: 200 ml/m3 1 min spray, 50 min exposure time
Evonta EggClean® Prototype (Evonta, Dresden, Germany) Low energy electron flow Radiation 200 keV, 60 kGy 1 s radiation
1+1 Wofasteril SC super (Kesla Hygiene AG, Bitterfeld-Wolfen, Germany) Peracetic acid in micro cages Foam 0.5% (1 ml Wofasteril, 1 ml Alcapur, 198 ml distilled water) 60 min exposure time, followed by washing with PBS
Vitasan® Spray (EW Nutrition, GmbH, Visbek, Germany) Essential oils Spray 5% (5 ml product with 95 ml distilled water) 20 min exposure time
Vitasan® Spray (EW Nutrition, GmbH, Visbek, Germany) Essential oils Fogging 5% (5 ml product with 95 ml distilled water) 6 min fogging, 20 min exposure time