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. 2020 May 14;15(5):e0232825. doi: 10.1371/journal.pone.0232825

Table 3. Humane endpoints–definition of criteria and scoring.

Signs—individuals Score
Ruffled, untended plumage A
Non-recurring sticky or soiled cloacal region A
Mild lameness (weight is still put on the affected limb) B
Distension of the abdomen B
Mildly intensified respiration, increased respiratory rate or open-beak breathing B
Mild/superficial lesion B
Automutilation and/or loss of feathers B
Sunken eyes B
Severe lameness, no weight is put on the affected limb C
Blood at body orifices, bloody feces C
Moderately to severely intensified respiration, wheezing, other respiratory sounds C
Longer lasting sticky or soiled cloacal region (over several observation intervals) C
Seizures, ataxia, torticollis, opisthotonus C
Apathy/moderately to severely reduced general condition (animal can no longer be mobilized, eyes stay closed upon approach/touching) C
Abscesses C
Visible malformations C
Lesions with poor prognosis C
Signs—flock Score
Feces deviate in amount, colour and texture A
Reduced or increased feed or water intake A
Abnormal feces: occurrence of distinctly wet litter A
Irregular distribution of animals in the pen (clustered in the centre or in the periphery) A
Divergence in body weight: Occurrence of individuals with less than 50% of the mean body weight of the flock B
Remarkable vocal expression B
Cannibalism C