Table 6. Trial 2 –Repeat formaldehyde disinfection, breeding and necropsy results.
| Group | A: Contaminated, treated | B: Contaminated, untreated | C: Control | |||
|---|---|---|---|---|---|---|
| Infertile eggs/early embryonic death [%] | 0 | 5.7 | 1.4 | |||
| Late embryonic death [%] | 2.9 | 0 | 0 | |||
| Hatchability [%] | 96 | 90 | 96 | |||
| Mortality [%] | 2.1 | 0 | 2.1 | |||
| Day 7 / 14 | Day 7 / 14 | Day 7 / 14 | ||||
| N | 24 | 23 | 23 | 22 | 24 | 23 |
| Mean body weight [g] | 214.3 | 538.0 | 231.5 | 545.4 | 217.5 | 549.0 |
| Mean relative liver weight [%] | 5.0 | 5.0a* | 4.7 | 3.7a* | 4.6 | 3.8 |
| Mean relative gut weight [%] | 11.0a* | 9.0 | 10.9 | 9.2 | 10.5a* | 9.1 |
| Macroscopy | ||||||
| Abnormal yolk sacs [n] | 7 | 3 | 9 | 5 | 3 | 1 |
| Abnormal kidneys [n] | 0 | 0 | 0 | 1 | 0 | 1 |
| Histology | ||||||
| Liver, extramedullary hematopoiesis [n] | 9 | 10a* | 11 | 10 | 5 | 3a* |
| Liver, lymphoid follicles [n] | 3 | 5a* | 3 | 9 | 7 | 14a* |
| Lung, lymphoid follicles [n] | 0a**,b* | 9 | 7a** | 14 | 5b* | 12 |
| Kidneys, lymphoid follicles [n] | 2 | 3 | 0 | 3 | 2 | 1 |
Statistical evaluation conducted only between group A to group B and C
a, b, c, d Superscripts within the row and time indicate significant differences
* indicates p ≤ 0.05 (significant)
** indicates p ≤ 0.01 (highly significant)