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. 2020 May 14;15(5):e0232825. doi: 10.1371/journal.pone.0232825

Table 11. Trial 7 –Essential oils ultrafogger disinfection, breeding and necropsy results.

Group A: Contaminated, treated B: Contaminated, untreated C: Control
Infertile eggs/early embryonic death [%] 0 1.4 4.3
Late embryonic death [%] 1.4 2.9 2.9
Hatchability [%] 98 98 90
Mortality [%] 4.1 2.0 0
Day 7 / 14 Day 7 / 14 Day 7 / 14
N 24 23 24 24 23 22
Mean body weight [g] 156.0a** 512.3b**,c* 185.4a** 460.0b** 168.9 471.7c*
Mean relative liver weight [%] 5.3 3.9a* 5.5 4.1a* 5.4 4.1
Mean relative gut weight [%] 12.9a**,b** 9.1c**,d** 10.7a** 8.4c** 11.8b** 9.7d**
Macroscopy
Abnormal yolk sacs [n] 8a** 9 3 11 0a** 10
Abnormal kidneys [n] 1 0 1 0 0 0
Histology
Liver, extramedullary hematopoiesis [n] 4 8a* 6 2 3 3a*
Liver, lymphoid follicles [n] 5 5a* 5 14a* 7 10
Lung, lymphoid follicles [n] 0 16 3 18 2 17
Kidneys, lymphoid follicles [n] 2 4 2 4 0 3

Statistical evaluation conducted only between group A to group B and C

a, b, c, d Superscripts within the row and time indicate significant differences

* indicates p ≤ 0.05 (significant)

** indicates p ≤ 0.01 (highly significant)