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. 2020 May 14;15(5):e0233075. doi: 10.1371/journal.pone.0233075

Fig 5. Cell morphology and detection of limbal stem cell marker, ΔNp63α.

Fig 5

(A) Representative confluent cell culture and (B) indirect immunofluorescence detection of the cells expressing both ΔNp63 (red) and p63α (green) that represent ΔNp63α-positive limbal stem cells (yellow) in outgrowth cultures from Lconj, Lm, and Lcor (200× magnification, scale bar = 100 μm). (C) 200× magnification (zoom in) of the stained cells from Lm (scale bar = 25 μm); colocalization of ΔNp63 (red) and p63α (green) was detected in the nuclei (shown in yellow and white arrows). (D) The percent of ΔNp63α-positive cells from Lconj, Lm, and Lcor outgrowth cultures. The line represents the mean percentage of the ΔNp63α-positive cells. Ns, not significant by ANOVA for repeated measures.