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. 2020 May 14;15(5):e0227720. doi: 10.1371/journal.pone.0227720

Fig 3. Complete Nr1d1 ablation induces catabolism of skeletal muscle in older mice.

Fig 3

(a) Heat-map showing the RT-qPCR quantified expression pattern of muscle catabolism genes Tgfb, Foxo3a, Smad2, and p38Mapk in the soleus muscle of 15-week-old Nr1d1+/+, Nr1d1+/- and Nr1d1-/- mice. (b) Mean expression of differentially-regulated catabolic factors in the soleus muscle of 15-week-old Nr1d1+/+, Nr1d1+/- and Nr1d1-/- mice shown in (n = 6 error bars +/- stdev) (c) Heat-map showing the RT-qPCR expression pattern of muscle anabolic genes mTor and Akt1-3 in the TA muscle of 15-week-old Nr1d1+/+, Nr1d1+/- and Nr1d1-/-. (d) RT-qPCR-quantified expression of Akt3 in the soleus muscle of 15-week-old Nr1d1+/+, Nr1d1+/- and Nr1d1-/- mice (n = 6 error bars +/- stdev). (e) Heat-map showing the RT-qPCR quantified expression pattern of muscle glycolytic enzymes Eno3, Tpi1, Hk1, Hk2, Pfkfb1, Pfkfb4, Glut4 and Pkm in the soleus muscle of 15-week-old Nr1d1+/+, Nr1d1+/- and Nr1d1-/- mice. (f) Mean expression of differentially-regulated glycolytic enzymes in the soleus muscle of 15-week-old Nr1d1+/- mice shown in (n = 6 error bars +/- stdev) (g) Immunoblot showing expression of MHC1A (slow myosin heavy chain), MHC2B (fast myosin heavy chain), FOXO3A, P38MAPK, AKT2 and ß-Actin (h) Fast myosin heavy chains in the TA muscle of 15 week old Nr1d1-/- mice (n = 3). *p<0.05 and **p<0.01 were determined by a One-Way ANOVA. RT-qPCR data are expressed as mean ± s.e.m.