Fig 8. Proteomic evaluation of the parent relative to the ittA mutant.

Tandem Mass Tags (TMT) was used to determine the relative abundance of the total proteome of three biological replicates of parent and ittA mutant strains grown under conditions that induce proteins important during mammalian infection. Volcano plot depicts log₂ fold change (x-axis) and log₁₀ adjusted p-value (y-axis) of proteins identified from parent versus the ittA sRNA mutant strain. Single proteins are plotted as dots. Proteins outside of the red dashed boxes are significant. Red spots have a +/- 2 fold change difference in the parent strain relative to the ittA mutant strain and a p-value < 0.05. Yellow spots are proteins that achieved significance of p-value < 0.05 and a fold change range between +/- 1.4 to 1.9. OspD and Oms28 were found to be significantly higher in abundance in the parent relative to the ittA mutant.