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. 2020 May 4;16(5):e1008188. doi: 10.1371/journal.ppat.1008188

Fig 5. SPOP deficiency attenuates resistance against Salmonella infection in mice.

Fig 5

Spop F/FCreERT2+/+ knockout mice were generated by Cre-LoxP recombination. Conditional knockout mice were intraperitoneally injected with tamoxifen three times every other day at a dose of 175mg/kg body weight. (A) Mice were challenged with Salmonella typhimurium and their survival rates monitored for 36 hours (n = 7 each group; statistical analysis: Mantel–Cox test). (B) Bacterial load in the livers of Spop-/-CreERT2 and Spop+/+CreERT2 mice injected intraperitoneally with Salmonella typhimurium. (C), (D), and (E) ELISA for IL-1β, IFN-α and IL-6 in the serum from Spop+/+CreERT2 and Spop-/-CreERT2 mice challenged with Salmonella typhymurium for 6 h (n = 5 per genotype). **p < 0.01 and error bars reflect ±SD.