Table 1. pMHC stability, P14 TCR affinity and thermodynamic parameters of gp33 variants.
| Peptide | gp33 | V3P | Y4F | V3P_Y4F |
|---|---|---|---|---|
| Sequence | KAVYNFATM | KAPYNFATM | KAVFNFATM | KAPFNFATM |
| Tm (C)a | 53.7±0.1 | 57.3±0.4 | 52.7±0.4 | 56.1±1.1 |
| KD (μM)b | 8.6±0.4 | 5.6±0.4 | ND | 80.3±22 |
| KD (μM)c | 5.8±2.4 | 1.1±0.2 | - | - |
| TΔS (kcal/mol)c | -3.1±1.0 | -0.5±0.3 | - | - |
| ΔH (kcal/mol)c | -10.2±0.7 | -8.5±0.5 | - | - |
a Measured with Circular Dichroism where Tm is the temperature with 50% pMHC denaturation.
b Determined from steady state SPR data with software BIAevaluation.
c Measured using ITC200 at 25C. ND–Not Determinable. The values are an average of at least two independent experiments ±SD