Fig 1. Chitin binding and endochitinase functions of ChiA.
(A) Schematic representation of ChiA with domain boundaries annotated. (B) ChiA-FL, subdomains (NT, N1, N2, N3, CTD) and ChiA-CTD mutants (E543M, Q583A, Q617A) were assayed for chitinase activity against p-NP-[GlcNAc]3. Data represent the mean and standard deviation for triplicate experiments. (C) Chitin pull-down experiment to assess direct interactions between immobilized chitin and ChiA. ChiA-FL and subdomains (NT, N1, N2, N3, CTD, CTDE543M) were incubated with chitin beads and analysed by SDS-PAGE. BSA was used as a control. L: loaded sample; B: eluted beads. Eluted samples undergo an upward shift compared to the input sample due to differences in buffer conditions. Data is representative of three independent repeat experiments.