FIGURE 11.

Sympathetic denervation leads to increased expression of motor denervation genes and miR-206. qPCR analysis of gene expression shows increased myogenin, Fbxo32, and Chrng at day 7 (A) while MuRF1, Fbxo32, Hdac4, and Chrng were increased at day 3 (B), after sympathectomy. FoxO1, FoxO3, and Gadd45a were not modified at either timepoint in GA or TA muscles. miR-206 expression increases in the TA muscle and serum levels 7 days after sympathectomy. N = 4 TA and GA muscles from four mice per group (C). The results are presented as fold changes relative to muscles or serum from sham mice (set at onefold). qPCR analysis of changes in Myogenin, Fbxo32, and Chrng gene expression were significant at day 7 after sympathectomy (A). P values for differences in MuRF1, Hdac4, Fbxo32, Chrng, and Chrna1 expression were also significant for the GA and TA, except for Chrna1 in the TA, muscle 3 days after sympathectomy (B). FoxO1, FoxO3, and Gadd45a were not significantly modified in GA or TA muscles at either timepoint. MiR-206 expression increased in the TA muscle and serum levels 7 days after sympathectomy. The P value for the GA muscle was not significant. *P < 0.05, ^†P < 0.01 and ^‡P < 0.001