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. Author manuscript; available in PMC: 2020 May 14.
Published in final edited form as: Acta Physiol (Oxf). 2018 Oct 22;225(3):e13195. doi: 10.1111/apha.13195

FIGURE 5.

FIGURE 5

Sympathectomy induces NF-H and NF-M dephosphorylation and increased levels of PP2A and PP1 phosphatases. Levels of NF-H (A), NF-M (B), NF-L (C), phosphorylated NF-H and NF-M (D), and PP2A (E) and PP1 phosphatases (F) in the lysates of both sciatic-tibio-peroneal nerves from three sham and three surgically sympathectomized mice. Blots and SDS PAGE were probed for GAPDH and Commassie blue (G, H) respectively. Quantification of the NF immunoblots normalized to GAPDH show a significant increase in NF-H and NF-M but not NF-H-L (I) with sympathectomy. Phosphorylated/non-phosphorylated ratio show decreased phosphorylation of both NF-H and NF-M subunits with sympathectomy. Levels of PP2A (E) and PP1 (F) phosphatases are significantly increased with sympathectomy expressed as a sym/sham ratio (K) or individual group measurements normalized to GAPDH (I). *P < 0.05, †P < 0.01 and ‡P < 0.001