Fig. 3. TRPM8-mediated alterations in IL-25 (A), IL-33 (B), and TSLP (C) mRNA levels in BEAS2B cells. The menthol-induced increase in the expression of each cytokine was attenuated by pretreatment with 10 μmol BCTC and 10 μmol dexamethasone. The data are presented as the means ± standard error of the means of 6 independent experiments.

TRPM8, transient receptor potential melastatin family member 8; IL, interleukin; TSLP, thymic stromal lymphopoietin; BCTC, N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl) piperazine-1-carboxamide.

Significant differences between untreated and 2-mM menthol-treated cells were analyzed by an unpaired t test (^*P < 0.05; ^†P < 0.01).