Fig. 4. Comparison of TRPM8 protein levels in supernatants and mRNA levels in cell lysates of sputum from asthmatic patients and normal controls. TRPM8 protein (A) and mRNA expression (B) were determined by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction, respectively. The data are presented as the mean ± standard error of the mean of each group (Mann-Whitney U test).

TRPM8, transient receptor potential melastatin family member 8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

^*P < 0.001.