Fig. 2. TLR4^−/− mice exhibited exacerbated airway remodeling after TDI exposure. (A) Representative immunohistochemical staining of MUC5AC in the lung sections of different groups. Original magnification was 200×. (B) Representative Masson trichrome-stained lung sections showing the collagen deposition of different groups. Original magnification was 200×. (C) Representative immunohistochemical staining of α-SMA indicates smooth muscle density. Original magnification was 200×. (D) Semi-quantification of MUC5AC staining was performed by ImageJ software (n = 4–6). (E) Semi-quantification of Masson staining was performed by ImageJ software (n = 4–6). (F) Semi-quantification of α-SMA staining was performed. The percentage of α-SMA positive staining area was measured by ImageJ software (n = 4–6).

WT, wild-type; TLR4, toll-like receptor 4; TDI, toluene diisocyanate; NS, not significant; MUC5AC, mucin-5AC; α-SMA, α-smooth muscle actin.

^*P < 0.01; ^† P < 0.001.