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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Dev Biol. 2020 Mar 10;462(1):85–100. doi: 10.1016/j.ydbio.2020.03.005

Figure 1: Live imaging of slow muscle cell migration concurrent with fast muscle fusion events.

Figure 1:

(A-F) Frames from a time-lapse movie (Supplemental Movie 1) of a 19 hpf six1b:lyn-GFP (green); smyhc1:lyn-tdTomato (magenta) double transgenic embryo. (A’-F’) Pseudo-colored images show the slow muscle channel (white) from frames (A-F) overlaid with the shape of two fast muscle precursors (green) as they interact with slow muscle cells and extend anteriorly. Dotted outlines in A’ show somite boundaries (yellow). (G-N) Frames from a time-lapse movie of a 20 hpf six1b:lyn-GFP (green); smyhc1:lyn-tdTomato (blue) double transgenic embryo injected with mRNA encoding H2B-CFP (fuchsia). The movie (Supplemental Movie 2), which spans 92.5 minutes, was taken when fusion is actively occurring. (G’-N’) Illustrations depict a pair of fusing cells from Supplemental Movie 2 (G-N; white arrowhead). The time stamp of each frame is indicated. Scale bars in F’ (for A-F’) and N (for G-N) are 20 μm.