Table 3.
sCT binding kinetic parameters from biolayer interferometry
| CTR.38–141 | Mean ± SEM | ||||
|---|---|---|---|---|---|
| N | kon (1 × 104/Ms) | koff (1/s) | Calculated KD (μM) | Steady state KD (μM) | |
| Endo H-treated WT | 3 | 17.8 ± 2.0 | 0.37 ± 0.02 | 2.1 ± 0.1 | 1.9 ± 0.2 |
| Endo H-treated N130D | 3 | 6.3 ± 1.0a | 0.73 ± 0.03a | 12.4 ± 2.5a | 11.9 ± 1.7a |
| Glycan-free WT | 3 | 2.1 ± 0.5a | 0.61 ± 0.03a | 30.9 ± 5.3a | 10.8 ± 2.2a |
All kinetic parameters were produced from ForteBio data analysis software (version 8.1).
a
p < 0.05 compared to Endo H-treated CTR.38–141 WT. ANOVA with Tukey’s post hoc test was used for statistical analysis.