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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Orthop Res. 2019 Dec 25;38(6):1257–1267. doi: 10.1002/jor.24567

Figure 1. Experimental methods.

Figure 1.

(a) Cylindrical explants were dissected from medial condyles of bovine stifles and bisected to created paired samples. (b) Samples were stained with a 3-color assay for mitochondrial polarity and mounted to the stable backplate of a custom impactor. This test frame was subsequently mounted on a confocal microscope. (c) Paired hemi-cylindrical samples were mounted side-by-side on the backplate such that the two were in the same fluid bath but only one was impacted, while the second served as a non-impacted control. Samples were imaged at various locations relative to the impact (8 square fields of view; 5 on the impacted sample, 3 on the non-impacted sample). (d) Samples were imaged at all locations longitudinally, including before and up to 60 minutes after impact. For treated samples, SS-31 peptide treatment was added to the PBS bath surrounding both hemi-cylinders 30 minutes before the first image.