Fig. 3.
Syncrip selectively stabilises the proslong isoforms in neurons. (A) Syncrip (Syp) protein is expressed in the type I NB (marked with Dpn) and progeny lineage in third instar larvae. Syp is also expressed in the neurons, where Pros is upregulated. (B) Western blot confirms Syp is immunoprecipitated (IP) selectively and efficiently. α-Tubulin (Tub) was used as negative control. (C) pros mRNA is enriched by Syp IP (58.3% of input pulled down) but not by IgG control IP (1.3%). Negative control, ribosomal rp49 (α-Syp: 3.2%, IgG: 0.9%) (n=7). Error bars represent s.e.m. (D) Loss of Syp has a minimal effect on transcription levels indicated by pros intron, but pros exon signal and Pros protein are greatly reduced. NBs outlined in white. (E) Intensity of pros intron signal in wild type and syp mutant. Normalised to wild type for each experiment. Significance was calculated using t-tests. **P<0.01, ns=not significant P>0.05. NB, sum of intensity of two transcription foci; progeny, intensity of single spot including both transcription foci. Scale bars: 10 µm.