Fig. 4.

Syncrip selectively stabilises the pros^long isoforms in neurons. (A) smFISH against pros long intron and pros long UTR shows that pros^long is normally transcribed in the syp mutant. (B) RNA sequencing shows selective loss of pros^long isoform in syp mutants. Read coverage for the first 3 kb of pros 3′ UTR is not different between wild type (wt) and syp mutant whereas the distal 12 kb of pros 3′ UTR is reduced in syp mutants. (C) Quantitative analysis of total sequencing reads of pros 3′ UTR in wild type and syp mutants. (D) Northern blot showing the selective loss of pros^long isoforms in syp mutants. The intensity of the 6.5 kb and 9 kb bands are similar between wild type and syp mutants (blue asterisks) whereas the 21 kb band is reduced in syp mutants (red asterisk). Scale bars: 10 μm.