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Syp and proslong are not required to maintain larval neurons in differentiated states. Clonal analysis shows normal GMC differentiation in the syp mutant. Type I NB lineage clones are marked with mCD8::GFP (outlined with white trace) and stained with IF against Pros, Dpn (wild type n=7, syp n=9) and Elav (wild type n=16, syp n=4). Both wild type and syp mutant clones have a single Dpn-expressing cell per clone and the progeny cells express Elav, marking a neuronal fate.
