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proslong-REDr terminates transcription of the pros UTR extension. Insertion of a dsRED marker in reverse orientation downstream of the 3 kb UTR termination signal (proslong-REDr) results in loss of the pros UTR extension (visualised with the pros long UTR smFISH probe). The pros exon smFISH shows that proslong-REDr expresses higher levels of pros than syp mutant brains, but much less than wild type. Scale bars: 20 μm.
