Fig. 7.

Loss of pros^long blocks Pros protein upregulation but has no effect on NB termination. (A) Pros protein level is not upregulated in the neurons of proslong-REDr larval brains. (B) Dpn marks NBs in pupal brains, 48 h APF. In wild type, only the four MB NBs remain. In the syp mutant, many NBs are long-lived in the pupal brain. In the proslong-REDr brains, type I NBs terminate normally and only the four MB NBs persist. (C) FasII IF stains γ and α/β neurons in the MB of 1-day old adult brains. In the syp RNAi knockdown brains there is loss of α/β neurons and excess γ neurons. There is no difference in MB morphology in the proslong-REDr line, compared to wild type.