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. 2020 Apr 20;40(4):573–579. [Article in Chinese] doi: 10.12122/j.issn.1673-4254.2020.04.20

1.

Real time PCR引物序列表

Primers used for real-time PCR

Gene Primer sequence
RANK: receptor activator of nuclear factor-κB ligand; NFATc1: Recombinant nuclear factor of activated T-cells, cytoplasmic 1; TRAF-6: Tumor necrosis factor receptor- associated factor-6; TRAP: Tartrate resistant acid phosphatase; CTSK: Cathepsin K.
GAPDH
Forward: GGTTGTCTCCTGCGACTTCA
Reverse: TGGTCCAGGGTTTCTTACTCC
RANK
Forward: AGCCTCCGAGCAGAACTGACTC
Reverse: CTGCCTGTGTAGCCATCTGTTGAG
NFATC-1
Forward: GGCTGGTCTTCCGAGTTCACATC
Reverse: GCTGTCTGTGCTCTGCTTCTCC
TRAF-6
Forward: AGGAATCACTTGGCACGACACTTG
Reverse: TGGTCCTGTCTTACTAGGCGACTC
TRAP
Forward: CCACCCTGAGATTTGTGGCT
Reverse: ACATACCAGGGGATGTTGCG
CTSK
Forward: CTGGAGGGCCAACTCAAGA
Reverse: CCTCTGCATTTAGCTGCCTT