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. 2020 May 4;147(21):dev187369. doi: 10.1242/dev.187369

Fig. 5.

Fig. 5.

Esrp1 ablation in ectoderm results in altered expression of components of the Wnt signaling pathway and other signaling pathways implicated in cleft lip and/or cleft palate. (A) qRT-PCR validation of selected changes in total transcript levels in Esrp1−/− ectoderm as well as in adjacent mesenchyme. Error bars indicate s.d. Statistical significance for each comparison was determined by two-tailed t-test. *P<0.05. (B) GO and KEGG pathway enrichment for genes that are downregulated in Esrp1−/− ectoderm. (C) Whole-mount in situ hybridization of E11.5 embryos showing reduced expression of Shh and Wnt9b in epithelial cells of the developing face. (D) Whole-mount and tissue section in situ hybridization showing reduced expression of canonical Wnt target genes Lef1 and Axin2 in Esrp1−/− embryos.