Fig. 6.

Reduced Wnt signaling in Esrp1^−/− confirmed in-crosses with WNT/β-catenin signaling reporter mice. (A) Reduced activation of the TCF/Lef1-GFP reporter is observed in ectoderm and mesenchyme of Esrp1^−/− MNP, LNP and MXP. (B) Reduced expression of the Axin2-lacZ reporter in LNP and MXP in Esrp1^−/− embryos. For A and B, frontal sections are shown. Graphs show quantification of mean fluorescence intensity corrected for area in WT (n=3) and Esrp1^−/− embryos (n=3). Error bars indicate s.d. Statistical significance was determined by two-tailed t-test. *P<0.05. Scale bars: 20 µm.