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. 2020 Apr;9(2):280–287. doi: 10.21037/tlcr.2020.03.13

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2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Differential methylation markers in pN0/pN+ malignant tissue and plasma samples. (A) Volcano map of 878 significantly differential methylation markers that distinguish pN0/pN+ malignant tissue samples. Annotated genes are markers shared with pN+ plasma; (B) Heatmap of 878 differential methylation markers that distinguish pN0/pN+ malignant tissue samples. Samples are in columns and markers are in rows. The Manhattan distances were calculated between markers. The ward.D2 method was used for Hierarchical clustering of markers.