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. 2020 Apr;9(2):257–268. doi: 10.21037/tlcr.2020.03.04

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2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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A possibly IHC-positive sample (surgically resected from patient No. 2). (A) Hematoxylin and eosin (H & E) staining (×400); (B) IHC (×400); (C,D) mRNA-ISH (×400). (A) Moderate anthracosis and macrophages were seen by H & E staining. (B) All tumor cells were stained faintly by IHC. Stippling in alveolar macrophages and extracellular mucins coexisted with highly granular intensity. (C,D) Two different parts of TMAs assessed by mRNA-ISH are shown. (C) Some clusters represent overlapping anthracosis (arrowhead). (D) Clusters and dots were observed dominantly in tumor cells forming an acinus (arrow, a single dot; arrowhead, a cluster). Scale bar: 200 μm. IHC, immunohistochemistry; ISH, in situ hybridization; TMA, tissue microarray.