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. 2020 May 14;10(5):57. doi: 10.1038/s41408-020-0324-3

Fig. 2. Primary SS cells upregulate FOXP3FOXP3 after stimulation with SE.

Fig. 2

a PBMCs from Sézary syndrome (SS) patients were cultured in the presence of vehicle (PBS) or a pool of S. aureus enterotoxins (SE) (SEA, SEB, SEC2, SED, and SEI) for 24h. FOXP3 expression was determined by qPCR. In each sample, the level of FOXP3 mRNA was normalized to that of GAPDH mRNA, and it is depicted as fold change compared to samples with PBS with geometric mean and 95% confidence interval. Student’s t-test was applied on delta CT values, p<0.05 (n=12). b PBMCs from SS patients were cultured in the presence of vehicle (PBS) or a pool of SE (SEA, SEB, SEC2, SED, and SEI) for 24h, and then sorted by FACS into malignant and nonmalignant cells. FOXP3 expression was determined by qPCR. In each sample, the level of FOXP3 mRNA was normalized to that of GAPDH mRNA and it is depicted as fold change compared to samples treated with PBS only. c PBMCs from SS patients were cultured in the presence of vehicle (PBS), or with either a SE-positive supernatant (SA sup A) or a SE-negative supernatant (SA sup B) from clinical S. aureus isolates for 72h. FOXP3 expression was determined by qPCR. In each sample, the level of FOXP3 mRNA was normalized to that of GAPDH mRNA and it is depicted as fold change compared to samples with PBS (n=4). d PBMCs from three SS patients were cultured in the presence of vehicle (PBS) or SE for 24h. FOXP3 expression was determined by western blotting.