Table 1.
Identification results for bacteria and yeast (monomicrobial BSI)
| No. of true/false positives no. of true/false negatives | No. of true/false positives no. of true/false negatives | ||||
|---|---|---|---|---|---|
| Species (n = reference method ID) | FilmArray® | ePlex® | FilmArray® | ePlex® | Comments |
| ID of Gram-positive organisms | |||||
| S. aureus (n = 18) | 18/0 | 18/0 | 119/0 | 119/0 | |
| S. epidermidis (n = 35) | 35/0a | 35/2 | 102/0 | 100/0 | FilmArray® ID only on Staphylococcus spp. level, false ePlex® ID of S. hominis as S. epidermidis, false ePlex® ID of S. warneri as S. epidermidis |
| S. haemolyticus (n = 8) | 8/0a | 7/0 | 129/0 | 129/1 | FilmArray® ID only on Staphylococcus species level, no ePlex® ID for one S. haemolyticus isolate |
| S. hominis (n = 6) | 6/0a | 5/0 | 131/0 | 131/1 | FilmArray® and ePlex® ID only on Staphylococcus spp. level, false ePlex® ID of S. hominis as S. epidermidis |
| S. warneri (n = 2) | 1/0a | 1/0 | 135/1 | 135/1 | FilmArray® and ePlex® ID only on Staphylococcus spp. level, no FilmArray® ID for 1/2 isolates, false ePlex® ID of one S. warneri isolate as S. epidermidis |
| S. capitis (n = 2) | 2/0a | 2/0 | 135/0 | 135/0 | FilmArray® and ePlex® ID only on Staphylococcus spp. level |
| E. faecium (n = 4) | 4/0a | 4/2 | 133/0 | 131/0 | FilmArray® ID only on Enterococcus spp. level, false ePlex® ID for two E. faecalis isolates as E. faecium |
| E. faecalis (n = 5) | 5/0a | 3/0 | 132/0 | 132/2 | FilmArray® ID only on Enterococcus spp. level, false ePlex® ID for two E. faecalis isolates as E. faecium |
| S. pneumoniae (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| S. gallolyticus (n = 2) | 2/0 | 2/0 | 135/0 | 135/0 | FilmArray® and ePlex® ID only on Streptococcus spp. level |
| S. mitis group (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | FilmArray® and ePlex® ID only on Streptococcus spp. level |
| S. oralis (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | FilmArray® and ePlex® ID only on Streptococcus spp. level |
| S. anginosus (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | FilmArray® ID only on Streptococcus spp. level |
| Streptococcus sp. (group C) (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | FilmArray® and ePlex® ID only on Streptococcus spp. level |
| C. acnes (n = 5) | n/a | 5/0 | n/a | 132/0 | Not included in the FilmArray® panel |
| A. meyeri (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| Actinomyces sp. (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| P. faecalis (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| Lactobacillus sp. (n = 1) | n/a | 1/0 | n/a | 136/0 | Not included in the FilmArray® panel, ePlex® ID only on Lactobacillus spp. level |
| Corynebacterium sp. (n = 1) | n/a | 1/0 | n/a | 136/0 | Not included in the FilmArray® panel |
| A. parvulum (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| Total number of organisms (n = 98) | 86/0 | 89/4 | 50/1 | 39/5 | |
| ID of Gram-negative organisms | |||||
| E. coli (n = 15) | 15/0 | 15/0 | 122/0 | 122/0 | |
| K. pneumoniae/variicola (n = 5) | 5/0 | 5/0 | 132/0 | 132/0 | Two K. variicola identified as K. pneumoniae by FilmArray® and ePlex® |
| K. oxytoca (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| S. marcescens (n = 2) | 2/0 | 2/0 | 135/0 | 135/0 | |
| E. cloacae Komplex (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| E. ludwigii (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | FilmArray® and ePlex® ID as E. cloacae complex |
| P. aeruginosa (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| H. influenzae (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| M. liquefaciens (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex®panel |
| N. polysaccharea (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| C. canimorsus (n = 1)a | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex®panel; aonly identified by 16S-rRNA-PCR |
| B. fragilis (n = 2) | n/a | 2/0 | n/a | 135/0 | Not included in the FilmArray® panel |
| B. vulgatus/dorei (n = 1) | n/a | n/a | n/a | n/a | Not included in the FilmArray® and ePlex® panel |
| Total number of organisms (n = 33) | 27/0 | 29/0 | 110/0 | 108/0 | |
| ID of yeasts | |||||
| Candida albicans (n = 2) | 2/0 | 2/0 | 135/0 | 135/0 | |
| Candida glabrata (n = 2) | 2/0 | 2/0 | 135/0 | 135/0 | |
| Candida parapsilosis (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| Candida tropicalis (n = 1) | 1/0 | 1/0 | 136/0 | 136/0 | |
| Total number of organisms (n = 6) | 6 | 6 | 131/0 | 131/0 | |
aCoagulase-negative Staphylococcus spp. as well as Enterococcus spp. were detected on genus level only by the FilmArray®