Table 2.
Resistance genes identified for Gram-positive bacteria by FilmArray® and ePlex®
| Genotypic detection of methicillin resistance (mecA/mecC ) | ||||||
|---|---|---|---|---|---|---|
| Staphylococcus species (total n=66) | mecA in house PCR | mecC in house PCR | mecA FilmArray® | mecA ePlex® | mecC ePlex® | comment |
| S. aureus (n=18) | negative (n=18)a | n/a | negative (n=18) | negative (n=18) | negative (n=18) | |
| Coagulase-negative Staphylococcus spp. (n=48) | negative (n=15)b | n/a | negative (n=16) | negative (n=15) | negative (n=48) | False-negative mecA result by FilmArray® (n=1) |
| positive (n=33)c | positive (n=31) | positive (n=33) | No target detected by FilmArray® (n=1) | |||
| Genotypic detection of vancomycin resistance (vanA/vanB) | ||||||
| Enterococcus species (total n=9) | vanA/vanB in-house PCR | vanA/vanB FilmArray® | vanA/vanB ePlex® | comment | ||
| Enterococcus vancomycin sensitive (n= 8) | negative (n=8) | negative (n=8) | negative (n=8) | E. faecalis (n=5), E. faecium (n=3) | ||
| Enterococcus vancomycin resistant (n= 1) | positive van B (n=1) | positive vanA /vanB (n=1) | positive vanB (n=1) | vanB positive E. faecium isolate | ||
atested from blood culture (n=13), tested from culture (n=5)
btested from blood culture (n=11), tested from culture (n=4)
ctested from blood culture (n=18), tested from culture (n=15)