Table 3.
Resistance genes identified for Gram-positive bacteria by FilmArray® and ePlex®
| Genotypic detection of methicillin resistance (mecA/mecC) | ||||||
| Staphylococcus species (total n = 66) | mecA in house PCR | mecC in house PCR | mecA FilmArray® | mecA ePlex® | mecC ePlex® | Comment |
| S. aureus (n = 18) | Negative (n = 18)a | n/a | Negative (n = 18) | Negative (n = 18) | Negative (n = 18) | |
| Coagulase-negative Staphylococcus spp. (n = 48) | Negative (n = 15)b | n/a | Negative (n = 16) | Negative (n = 15) | Negative (n = 48) | False-negative mecA result by FilmArray® (n = 1) |
| Positive (n = 33)c | Positive (n = 31) | Positive (n = 33) | No target detected by FilmArray® (n = 1) | |||
| Genotypic detection of vancomycin resistance (vanA/vanB) | ||||||
| Enterococcus species (total n = 9) | vanA/vanB in-house PCR | vanA/vanB FilmArray® | vanA/vanB ePlex® | Comment | ||
| Enterococcus vancomycin sensitive (n = 8) | negative (n = 8) | negative (n = 8) | negative (n = 8) | E. faecalis (n = 5), E. faecium (n = 3) | ||
| Enterococcus vancomycin resistant (n = 1) | positive van B (n = 1) | positive vanA /vanB (n = 1) | positive vanB (n = 1) | vanB positive E. faecium isolate | ||
aTested from blood culture (n = 13), tested from culture (n = 5)
bTested from blood culture (n = 11), tested from culture (n = 4)
cTested from blood culture (n = 18), tested from culture (n = 15)