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Effect of the solvent in the quantification of HEPES. Each solution was analyzed by HPLC in triplicate
| Solvent | Retention time (min) | Concentration of HEPES (μg/mL) | t-test (p) |
|---|---|---|---|
| Matrix (10% EtOH/PBS) | 2.60 ± 0.01 | 9.35 ± 0.16 | ____ |
| NaCl 0.9% | 2.69 ± 0.01 | 9.35 ± 0.29 | 0.99 |
| PBS | 2.70 ± 0.01 | 9.98 ± 0.40 | 0.09 |
