Figure 2.

Effect of FVII depletion on platelet deposition during thrombus formation on tissue factor or collagen. Grafts were coated with either collagen [top row; (a, b)] or tissue factor [bottom row; (c, d)]. Platelet deposition was recorded within the graft [left column; (a, c)] as well as the downstream thrombus tail segment [right column; (b, d)]. The three treatment groups included control (circles, n = 6), enoxaparin (diamonds, n = 6), and FVII-depleted (ASO-treated) (triangles, n = 8). Graft experiments were performed 13–15 days after initiation of FVII–ASO treatment, when PT was prolonged ≥ 2.0 times the baseline, corresponding to 93–97% reduction in FVII antigen levels compared to baseline. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control.