Fig. 3.

Flow cytometric analysis of treated/untreated HSC4 and KDR cells. a–c Represent untreated, treated with cell-free supernatant (60 µL/mL), and treated with cisplatin (IC₅₀) at HSC4 cells and d–f represent untreated, treated with cell-free supernatant (60 µL/mL), and treated with cisplatin (IC50) at KDR cells respectively for 24 h incubation. Dots with Annexin V⁻/PI⁺ (Q1), Annexin V⁺/PI⁺ (Q2), Annexin V⁺/PI⁻ (Q3), and Annexin V⁻/PI⁻ (Q4) and feature represent necrotically, late apoptotic, early apoptotic, and viable intact cells respectively. All experiments were performed in triplicate